Dry-State Preservation

Like many of the working groups within IBRG, the Dry-State Preservation Group is comprised of representatives from various industries (eg paint and plastics), preservative manufacturers, testing institutes and independent testing laboratories and academic institutions. The projects arise from needs within the coatings industry and attempt to encompass both the latest understanding of the technology and to be in line with the most recent legislative requirements. The group is led by members of from industry to ensure relevance and acceptance of the methods the group develops, while ensuring the needs of the preservative industry and regulators are met.

Current activities include:

Dry-Film Fungal Testing

During the 1970's and early 1980's, the work of the IBRG Paints Group was focussed on investigations into fungal growth on paint films. From this and extensive ring tests a standard test protocol was developed which has gained wide acceptance in the paint industry and forms the basis of BS3900 Part G6. The method was validated by both laboratory studies and field trials and members of IBRG are providing input into the latest revision of the standard.

Dry-Film Algal Testing

As a continuation of the work on dry-film fungicides, IBRG is in the latter stages of developing a standard test protocol for the study of algal growth on painted surfaces. Like the current version of BS3900 Part G6, it is a panel test and is intended to help predict the performance of materials in use through the use of a standardised laboratory procedure. The method has been proposed for inclusion in the latest revision of BS3900 Part G6.

Plastics

The group is also active in other areas such as the review of ISO 846 and has an interest in developing approaches for materials such as wood-plastic composites. In previous years, IBRG produced the ground work and provided the international ring test data for the 1997 revision of ISO 846 as well as a number of methods associated with biodegradable plastics. This lead to improved definitions and work was performed to provide the data in support of ISO 14851 and ISO 14852.

To help measure the efficacy of biocides used to protect plastics from spoilage and degradation by fungi, the group developed a thin agar overlay method for measuring fungistatic activity. After a series of ring tests, this was published as ISO 16869. It is likely that the group will return to this application in the near future to provide methods that reflect normal usage of plastics, possibly in association with work on wood-plastic composites.

TOPTOP

Further Details:

Further details of the work of the IBRG Dry-State Preservation Group can be obtained from the General Secretary of IBRG who is also the Secretary of the Working Group:-
Gillian Iredale, IMSL, Pale Lane, Hartley Wintney Hampshire, RG27 8DH, UK
Telephone: +44 (0)1252 627676
Telefax: +44 (0)1252 627678
E-mail: <click-here>

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  • £100.00
    Determining the Resistance of Paints and Coatings to Algal Growth
    This document describes a method to determine the resistance of paints and coatings to the growth of photosynthetic microorganisms and the effectiveness of biocides (preservatives) that might be used in them. Samples are inoculated with defined suspensions of microorganisms and then incubated. The changes in the size of the populations both over time and in treated compared with untreated materials are used to describe the basic antimicrobial properties of the materials. The results obtained may not describe the performance of the treated materials under normal conditions of use.
    Add
    IBRG DSP19-004
  • £100.00
    Determining the Resistance of Paints and Coatings to Fungal Growth
    This document describes a method to determine the resistance of paints and coatings to the growth of Fungi and the effectiveness of biocides (preservatives) that might be used in them. Samples are inoculated with defined suspensions of microorganisms and then incubated. The changes in the size of the populations both over time and in treated compared with untreated materials are used to describe the basic antimicrobial properties of the materials. The results obtained may not describe the performance of the treated materials under normal conditions of use.
    Add
    IBRG DSP20-001
    • Peter Askew 2014 Efficacy Assessment of Treated Articles - A guidance Nordic Council
      http://www.norden.org/en/publications/pu...Abstract...
      This report provides guidance how to test efficacy of articles or material treated with biocides. There are two major groups: The first where the treatment is intended to protect the article itself. To prove efficacy, the material/article has to be tested against an untreated variant. In the second group, the treatment is intended to introduce new properties, e.g. to provide hygienic or insect repellency-functions. The problem which the treatment is intended to solve needs to be shown on the untreated article under normal conditions of use. This way, the benefit of the treatment can be shown.
    • Lunenberg-Duindam J, Lindner W 2000 In-can Preservation of Emulsion Paints European Coatings Journal 03 66-73 Vincentz
      Abstract...
      A microbiological test method for evaluation of the wet-state resistance of water based paints to bacteria has been developed. In a series of collaborative tests starting in 1978 many aspects of in-can-preservation were investigated. These have culminated in a round robin test (phase VII) designed to compare the precision of viable count and streak plate methods as a means of determining bacterial populations in well-preserved, unpreserved and so-called border line preserved paints. Reproducibility and repeatablity of both methods are reported. The streak plate method was found to be adequate to recover surviving or growing bacteria from the challenged paint samples. Good correlations of the streak results to log viable counts were found.
    • Philipp Stiefel, Jana Schneider, Caroline Amberg, Katharina Maniura-Weber & Qun Ren 2016 A simple and rapid method for optical visualization and quantification of bacteria on textiles Scientific RepoRts | 6:39635 | DOI: 10.1038/srep39 6
      https://www.ncbi.nlm.nih.gov/pmc/article...Abstract...
      To prevent bacterial contamination on textiles and the associated undesired effects different biocidal coatings have been investigated and applied. However, due to health and environmental concerns anti-adhesive coatings preventing the binding of bacteria would be favored. To develop such anti- adhesive coatings simple assays for reliable and fast screening are beneficial. Here an easy-to-handle, robust and rapid assay to assess bacteria on textiles utilizing a tetrazolium salt was reported. The assay allowed direct eye visualization of the color change of the textiles containing bacteria, facilitating fast screening. Quantification of the adhered bacteria could be done by generating standard curves which correlate the staining intensity to cell numbers. An additional advantage of the described assay is that with the same detection method anti-adhesive and biocidal effects can be investigated. The method was applied to different coatings, using Pseudomonas aeruginosa and Staphylococcus aureus as model organisms. The detection limit was found to be between 2.5 * 106 and 9.4 * 108 for P. aeruginosa and between 1 * 106 and 3.3 * 108 for S. aureus. The anti-adhesive coating PLUMA was demonstrated to reduce bacterial adhesion without killing them, whereas the biocidal coating TH22-27 caused a clear reduction in the number of viable cells.
    • Johannes Karl-Mark Knobloch, Sabrina Tofern, Wladimir Kunz, Sara Schütze, Michael Riecke, Werner Solbach, Thomas Wuske 2017 “Life-like” assessment of antimicrobial surfaces by a new touch transfer assay displays strong superiority of a copper alloy compared to silver containing surfaces PLOS ONE | https://doi.org/10.1371/journal.pone.01 10
      https://journals.plos.org/plosone/articl...Abstract...
      Transmission of bacteria from inanimate surfaces in healthcare associated environments is an important source of hospital acquired infections. A number of commercially available medical devices promise to fulfill antibacterial activity to reduce environmental contamina- tion. In this study we developed a touch transfer assay modeling fingerprint transmission to investigate the antibacterial activity of surfaces, with confirmed antibacterial activity by a modified ISO 22196 (JIS Z 2801) assay to test such surfaces under more realistic condi- tions. Bacteria were taken up from a dry standardized primary contaminated surface (PCS) with disinfected fingers or fingers covered with sterile and moistened cotton gloves. Subse- quently, bacteria were transferred by pressing on secondary contaminated surfaces (SCS) with or without potential antibacterial activity and the relative reduction rate was determined after 24 h. A stable transmission rate between PCS and SCS was observed using moist- ened sterile gloves. A copper containing alloy displayed at least a tenfold reduction of the bacterial load consistently reaching less than 2.5 cfu/cm2. In contrast, no significant reduc- tion of bacterial contamination by silver containing surfaces and matured pure silver was observed in the touch transfer assay. With the touch transfer assay we successfully estab- lished a new reproducible method modeling cross contamination. Using the new method we were able to demonstrate that several surfaces with confirmed antimicrobial activity in a modified ISO 22196 (JIS Z 2801) assay lacked effectiveness under defined ambient condi- tions. This data indicate that liquid based assays like the ISO 22196 should be critically reviewed before claiming antibacterial activity for surfaces in the setting of contamination of dry surfaces by contact to the human skin. We suggest the newly developed touch transfer assay as a new additional tool for the assessment of potential antimicrobial surfaces prior utilization in hospital environments.
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